Details for An, L., J. Hu, M. Yang, B. Zheng, A. Wei, J. Shang and X. Zhao, 2011
 
Citation An, L., J. Hu, M. Yang, B. Zheng, A. Wei, J. Shang and X. Zhao, 2011. CYP1A mRNA expression in redeye mullets (Liza haematocheila) from Bohai Bay, China. Marine Pollution Bulletin 62(4):718-725.
DOI / ISBN
Paper URL http://www.sciencedirect.com/science/article/pii/S0025326X11000233
Ref. No. 114075
Language English
Usage used in part
Remarks Abstract Induction of cytochrome P4501A (CYP1A) has been used as a biomarker in fish for monitoring aromatic and organic contaminants. In this study, a partial of CYP1A gene in redeye mullet (Liza haematocheila) was isolated and sequenced, and then a real-time quantitative reverse-transcription polymerase chain reaction assay was developed for quantification of CYP1A mRNA normalized to β-actin. The developed method was applied to detect CYP1A mRNA expression in redeye mullets collected from Nandaihe (reference site) and Dashentang (impacted site) in Bohai Bay, China. CYP1A mRNA expression values were significantly elevated in redeye mullets from Dashentang compared to a reference site – Nandaihe, which was correlated with the contents of different environmentally relevant pollutants in tissues, particularly with PCBs and PBDEs.
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